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The production of sorbose from sorbitol in resting cell system of Acetobacter suboxydans was studied. The conversion of sorbose from sorbitol was markedly influenced by several factors such as the substrate concentration, reaction time, temperature, pH. metal ions, growth factors and aeration in the resting cells. Sorbose production rapidly increased in the range of 6 ㎎/㎖ cells with the concentration of 5% sorbitol. For production of sorbose from sorbitol, optimal temperature and pH were 30℃ and 6.0. The production of sorbose from sorbitol was activated by 1 mM of Al^(+3) while inhibited by Ni^(+2). The conversion of sorbitol to sorbose was stimulated by the adding of 1 mM p-aminobenzoic acid and nicotinic acid, respectively. During incubation of 1.5 ㎖ of reaction mixture in 50 ㎖ of Erlenmeyer flask, 5% sorbitol was completely converted to sorbose after 20 hours.
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